Research Team of INSERM U964
Dr. Jean-Marc EGLY and Dr. Frédéric COIN
Our objectif: Understand the molecular mechanisms of Gene expression and DNA repair
Our model: Several Genetic Disorders that predispose patients to cancer and ageing
Our Research Area: Cancer and Ageing
Our Location: Institut de Génétique et de Biologie Moléculaire and Cellulaire (IGBMC, Illkirch, France)
Transcription is one of the key steps of gene expression in response to different stimuli of the organism like stress or hormones. Corect gene expression requires a combination of factors. The deleterious action of physical or chemical agents that create lesions in the DNA disrupts the expression of genes. If these lesions are not removed by efficient repair systems they will be at the origin of mutations that can lead to cancer and aging. TFIIH, a multi-subunit complex that we are studying plays a pivotal role in both the transcription of genes and their repair.
Main publications
Le May, N., Mota-Fernandes, D., Velez-Cruz, R., Iltis, I., Biard, D., and Egly, J.M. NER factors are recruited to active promoters and facilitate chromatin modification for transcription in the absence of exogenous genotoxic attack. Mol Cell 38, 54-66.(2011)
http://www.ncbi.nlm.nih.gov/pubmed/20385089
How DNA repair factors participate to nuclear-receptor gene expression
Ueda, T., Compe, E., Catez, P., Kraemer, K.H., and Egly, J.M. Both XPD alleles contribute to the phenotype of compound heterozygote xeroderma pigmentosum patients.J Exp Med. 206:3031-3046. (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19934020
How the second allele in XP patients participates to the phenotype of XP patients
Coin, F., Oksenych, V., Mocquet, V., Groh, S., Blattner, C., and Egly, J.M. Nucleotide excision repair driven by the dissociation of CAK from TFIIH. Mol Cell. 31:9-20. (2008).
http://www.ncbi.nlm.nih.gov/pubmed/18614043
How the composition of TFIIH is dynamics. Featured Article
Coin, F., Oksenych, V., and Egly, J. M. (2007). Distinct Roles for the XPB/p52 and XPD/p44 Subcomplexes of TFIIH in Damaged DNA Opening during Nucleotide Excision Repair. Mol Cell 26, 245-256.
http://www.ncbi.nlm.nih.gov/pubmed/17466626
How the two helicases in TFIIH make the job, helped by TFIIH subunits
Compe, E., Malerba, M., Soler, L., Marescaux, J., Borrelli, E., and Egly, J.M. Neurological defects in trichothiodystrophy reveal a coactivator function of TFIIH. Nat Neurosci. 10:1414-1422. (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17952069
How TFIIH can be also a co-activator in transcription
Coin, F., De Santis, L.P., Nardo, T., Zlobinskaya, O., Stefanini, M., and Egly, J.M. p8/TTD-A as a Repair- Specific TFIIH Subunit. Mol Cell. 21:215-226. (2006).
http://www.ncbi.nlm.nih.gov/pubmed/16427011
How the last subunit of TFIIH participates specifically to DNA repair
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